One of the major problems in cancer biology is defining the downstream targets of altered genes in tumor cells. To address this issue, a novel technology, cDNA microarray hybridization is being developed to analyze the consequences of chromosome anomalies at the level of gene expression. Using a robotic device, it is possible to print thousands of cDNA clones on a single microscope slide. Fluorescent cDNA probes prepared from any cell or tissue source of interest are then hybridized to these arrays providing a large scale view of gene expression. The ultimate goal of this project is genome wide expression analysis. In this fashion, it will be possible to determine the consequences of a given genetic alteration on gene expression. This technology is now being applied in model systems carrying alterations in tumor specific genes affected by translocation, amplification or inactivation.

[Selected Publications] [Cancer Genetics Branch]